P-066. In-vitro maturation of bovine and human oocytes in a chemically defined medium supplemented with epidermal growth factor
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چکیده
منابع مشابه
P-136: In Vitro Development of Ovine Oocytes Matured in A Chemically Defined Medium
Background: Despite great advances achieved in the field of in vitro oocyte maturation (IVM), almost all IVM media are supplemented with undefined component which not only pose the risk of pathogen transmission but also hinders our knowledge of molecular mechanisms of maturation. One of these components is serum that containing various concentration of unknown molecules affecting cellular proce...
متن کاملIn vitro Maturation and Fertilization of Buffalo Oocytes Cultured in Media Supplemented with Bovine Serum Albumin
The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...
متن کاملeffects of selenium, calcium and calcium ionophore on human oocytes in vitro maturation in a chemically defined medium
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P-130: Efficacy of Commercial In Vitro Maturation Medium Is Similar to Home-made Medium for In Vitro Maturation of Human Oocytes
Background: In vitro maturation (IVM) of immature oocyte is a promising treatment option for infertile women.IVM procedures attempt to mimic the natural conditions of the follicular environment. The technique of IVM of oocytes has been successful after the first trial using culture of human oocytes in follicular fluid. The crucial part of this technique is the maturation process itself, and the...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1997
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/12.suppl_2.151